Denaturant Concentration Calculator

  1. Sosnick Group
  2. [GdmCl],[Urea] Calculator
[GdmCl] and [Urea] Calculator
Buffer 
Refractive Index
Denaturant 
Refractive Index
Warn me if above solubility (25oC)

           
[GdmCl]                               [Urea] 
Property
Urea
GdmCl
Molecular Weight
60.056 g/mol
95.533 g/mol
Solubility, 25oC
10.49 M
8.54 M
d/doa
1 + 0.2658W + 0.0330 W2
1 + 0.2710W + 0.0330 W2
Molarityb
117.66(Dh) + 29.753(Dh)2 + 185.56(Dh)3
57.147(Dh) + 38.68(Dh)2 - 91.60(Dh)3
Grams of Denaturant/Gram of H2O
    
6 M
0.495 g
1.009 g
8 M
0.755 g
1.816 g
10 M
1.103 g
--
aW is the weight fraction of denaturant in the solution, d is the density of the solution and do is the density of H2O.
bDh is the difference in refractive index between the denaturant solution and water (or buffer) at the sodium D line. The equation for urea solutions is based on data from Warren and Gordon, and the equation for GdmCl is from Nozaki.
 
Weight %
Moles/liter
Moles/kg H2O
4.720
0.500
--
5.693
0.603
0.632
10.299
1.105
1.202
20.052
2.209
2.625
23.767
2.645
3.263
29.377
3.318
4.354
34.688
3.975
5.559
38.197
4.418
6.469
42.247
4.940
7.656
42.700
5.000
--
46.741
5.531
9.186
50.040
5.973
10.484
50.236
6.000
--
52.791
6.348
11.704
54.759
6.619
12.669
54.847
6.632
12.714
57.528
7.005
14.177
60.340
7.399
15.925

Original tables of data for making denaturant solutions. [PDF]