Photoswitchable allosteric proteins

Photoswitchable allosteric proteins

Photoswitchable allosteric proteins

We have actively studied the biophysical dynamics in a photoswitchable protein (LOV2 domain) and used its photocycle to engineer light-controlled protein machines

Approach

    A.R. French, T.R. Sosnick, R.S. Rock, "Investigations of human myosin VI targeting using optogenetically controlled cargo loading" Proc Natl Acad Sci U S A 114 (2017) E1607-16.

    J.P. Zayner, T.R. Sosnick, "Factors that control the chemistry of the LOV domain photocycle" PLoS One 9 (2014) e87074.

    J.P. Zayner, C. Antoniou, A.R. French, R.J. Hause, Jr., T.R. Sosnick, "Investigating models of protein function and allostery with a widespread mutational analysis of a light-activated protein" Biophys J 105 (2013) 1027-36.

    D. Strickland, Y. Lin, E. Wagner, C.M. Hope, J. Zayner, C. Antoniou, T.R. Sosnick, E.L. Weiss, M. Glotzer, "TULIPs: tunable, light-controlled interacting protein tags for cell biology" Nat Methods 9 (2012) 379-84.

    J.P. Zayner, C. Antoniou, T.R. Sosnick, "The amino-terminal helix modulates light-activated conformational changes in AsLOV2" J Mol Biol 419 (2012) 61-74.

    D. Strickland, X. Yao, G. Gawlak, M.K. Rosen, K.H. Gardner, T.R. Sosnick, "Rationally improving LOV domain-based photoswitches" Nat Methods 7 (2010) 623-6.

    D. Strickland, K. Moffat, T.R. Sosnick, "Light-activated DNA binding in a designed allosteric protein" Proc Natl Acad Sci U S A 105 (2008) 10709-14.