Photoswitchable allosteric proteins

Photoswitchable allosteric proteins

Photoswitchable allosteric proteins

The Sosnick lab has been a leader in the development of light-sensitive protein engineering using LOV domains with effector proteins. This strategy can be used to control DNA binding, myosin VI, and co-localization with sub-cellular precision using light (Strickland et al., 2008, 2012; French et al., 2017). Integral to successful implementation is our characterization of the light-induced changes in the LOV2 protein (Zayner et al., 2012, 2013, 2014) that are coupled to the effector domain that can be tuned to provide the best light:dark response (Strickland et al., 2010).

Related video

Light-dependent recruitment of Myosin VI to peroxisomes

Show are sequential movies showing light-dependent recruitment of myosin VI to peroxisomes with subsequent release of myosin VI in the dark. Time resets to 0 s at the start of each video. (Scale bar, 5 μm). Video taken from Movie S2 of French et al., 2017.

A.R. French, T.R. Sosnick, R.S. Rock, "Investigations of human myosin VI targeting using optogenetically controlled cargo loading" Proc Natl Acad Sci U S A 114 (2017) E1607-16.

J.P. Zayner, T.R. Sosnick, "Factors that control the chemistry of the LOV domain photocycle" PLoS One 9 (2014) e87074.

J.P. Zayner, C. Antoniou, A.R. French, R.J. Hause, Jr., T.R. Sosnick, "Investigating models of protein function and allostery with a widespread mutational analysis of a light-activated protein" Biophys J 105 (2013) 1027-36.

D. Strickland, Y. Lin, E. Wagner, C.M. Hope, J. Zayner, C. Antoniou, T.R. Sosnick, E.L. Weiss, M. Glotzer, "TULIPs: tunable, light-controlled interacting protein tags for cell biology" Nat Methods 9 (2012) 379-84.

J.P. Zayner, C. Antoniou, T.R. Sosnick, "The amino-terminal helix modulates light-activated conformational changes in AsLOV2" J Mol Biol 419 (2012) 61-74.

D. Strickland, X. Yao, G. Gawlak, M.K. Rosen, K.H. Gardner, T.R. Sosnick, "Rationally improving LOV domain-based photoswitches" Nat Methods 7 (2010) 623-6.

D. Strickland, K. Moffat, T.R. Sosnick, "Light-activated DNA binding in a designed allosteric protein" Proc Natl Acad Sci U S A 105 (2008) 10709-14.