Thank you for choosing to use the Sosnick Lab's SAXSonIDPs server to interpret your SAXS data.
Our goal is to provide access to tools we have developed to extract information about the conformational
ensemble of disordered proteins and other flexible polymers, and to compare the results to methods
from the field of polymer physics.
Please cite our work which can be found at:
Riback JA, Bowman MA, Zmyslowski AM, Knoverek CR, Jumper JM, Hinshaw JR, Kaye EB, Freed KF, Clark PL*, Sosnick TR*. Innovative scattering analysis shows hydrophobic disordered proteins are expanded in water. (2017) Science. 358 (238-241)
To begin fitting your data, go to the section labeled Step 1: Select SAXS data to fit
Please contact Joshua Riback for more information, help fitting, or comments on the website.
If you have your own data accessible, choose it here:
The file format must contain contain q, I(q), and non-zero errors. Select configuration options below:
Units of q:
header lines to remove:
footer lines to remove:
Note: Since we fit the scattering profile out to large q, reliable fitting requires the data have a proper background subtracted.
You may fit our data to obtain an ideal of the expected deviations and the sensitivity of our fitting function.
Choose from example data
PNt 2M Gdn-334aa
PNt 4M Gdn-334aa
PNt 250mM Sarcosine-334aa
plug,FhuA 2M Gdn-144aa
reduced,RNaseA 2M Gdn-124aa
reduced,AFP 2M Gdn-81aa
To fit with current options click
otherwise proceed to the section labled Step 2: Select fit function, fit parameters, and output style
Polymer MFF (recommended)
Scaling Gaussian Coil Model
This section will contain warnings about each model and points to note. IF YOU SEE THIS WEBSITE IS NOT WORKING =(
Fitting is conducted using all points in the fitting range. To visually improve the data quality, we provide an option for rebinning the data with appropriate errors (recommended for publication).Bin?:
No. Show all points
No. Show all points
How many data points would you like to have shown every decade in q?
(Again, note this is for aesthetics, fitting is done BEFORE binning)
To compare with expected pre-factor R0 vs. ν trend for polypeptides (polymer MFF only), please provide the number of residues in your protein
Once you are done click to proceed.
(point calculated using given AA length and best fit parameters to polymer MFF)
Currently in development. Please contact us for this service.